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The nCounter technology at a glance

• Low input amounts required:
  o ≥ 50 ng total RNA for the analysis of mRNA, miRNA, lncRNA und miRGE
  o ≥ 300 ng fragmented DNA
  o≥ 10 ng ChIP DNA (not amplified)
  o Up to 8 μl of amplified single cell(s) or low input samples
  o ≥ 100,000 cells for protein analysis
  o ≥ 150,000 cells for RNA- and protein analysis
  o ≥ 100 mm2 tumor tissue area of a 10 μm thick FFPE slide for breast cancer diagnostics

• Up to 800-plex target detection
• Enzyme-free reactions (miRNA and miRGE assays require a ligase reaction; single cell analysis includes preamplificaiton steps)
• Suitable for low quality samples (e.g. FFPE)
• Highly sensitive (comparable to qPCR) and very high dynamic range combined with high reproducibility
• Digital analysis, self-validating
• Suitable for the validation of microarrays and biomarker screening studies
• Highly customisable assays
• In vitro diagnostic test (FDA- and CE-IVD certified) for patients with breast cancer (Prosigna ®)